In Vitro: FFA3 agonist 1 (0.01-100 µM) increases the incorporation of [35S]GTPgS and the generation of pERK1/2, reduces the production of cAMP in Flp-In T-REx 293 cells that induced the expression of enhanced yellow fluorescent protein (eYFP) human free fatty acid receptor 3 (hFFA3). FFA3 agonist 1 (0.1-100 µM) increases the incorporation of [35S]GTPgS in Flp-In T-REx 293 cells induced to express hFFA3, mouse FFA3 (mFFA3) and rat FFA3 (rFFA3).