In Vitro: Palmitoylation promotes the release of GSDME-C from GSDME-N after cleavage, and 2-BP inhibits the palmitoylation process, while inhibiting the modification of GSDME-C increases the interaction between GSDME-C and GSDME-N. 2-BP inhibits TNFa+CHX-induced apoptosis but not total cell death.