In Vitro: CYT296 (250nM, 72 h) changes the MEF chromatin and allows easier access of Yamanaka factors and facilitates the reprogramming process. CYT296 (250nM, 72 h) block heterochromatin assembly of MEFs to improve the generation of iPSC.
In Vivo: CYT296 (10-15 4F- and 2F -iPSC clones for subcutaneous injection, once) generates the iPSCs are pluripotent in the NOD-SCID mice.