产品名称：Recombinant Human EIF3S1/EIF3J Protein

规格或纯度：无载体, 生物活性, ActiBioPure™, ≥90%(SDS-PAGE), 见COA

生化机理：Eukaryotic translation initiation factor 3 subunit J (EIF3J) is a member of the EIF-3 subunit J family. EIF3 has a crucial role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to produce the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA access channel of the 40S subunit, setting EIF3J directly in the ribosomal decoding center. In addition, EIF3J interacts with eIF1A and reduces 40S subunit affinity for mRNA. An elevated affinity for mRNA is restored upon enlistment of initiator tRNA, despite the fact EIF3J remains in the mRNA-binding cleft in the presence of tRNA. Post-translational: Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

应用：SDS-PAGE, Functional Studies

生物活性：Immobilized Recombinant Human EIF3S1/EIF3J Protein (rp192076) at 1.0 μg/mL can bind EIF3S1/EIF3J Antibody (Ab101341) with the EC50 of 72.7 ng/mL.

氨基酸：70-258 aa

无载体：Yes

无动物源：No

Accession #：O75822

预测分子量：22.7 kDa

SDS-PAGE：26.6 kDa, under reducing conditions; 26.6 kDa, under non-reducing conditions.

级别：无载体, 生物活性, ActiBioPure™

储存缓冲液：20mM Tris, 200mM NaCl, 2mM DTT, 5% Glycerol, pH 8.0

浓度：见COA

储存温度：-20°C储存,避免反复冻融

运输条件：超低温冰袋运输

稳定性与储存：-20℃ 存储6个月；收货后建议分装，避免反复冻融。

Accession #：O75822

生物活性：Immobilized Recombinant Human EIF3S1/EIF3J Protein (rp192076) at 1.0 μg/mL can bind EIF3S1/EIF3J Antibody (Ab101341) with the EC50 of 72.7 ng/mL.

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