P128541-0.6KUPlasma Amine Oxidase from bovine plasma
面议
- 阿拉丁
- 生产商
- P128541-0.6KU
- 现货
- 全国
规格 |
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耗材描述
- 分子式
- 分子量
- Beilstein号
- EC号 232-623-0
- MDL号
- PubChem编号
属性
存贮条件 | 储存温度 -20°C |
描述
产品介绍 | Plasma amine oxidase (PAO) catalyzes the reaction:RCH2NH2 + O2 + H2O → RCHO + NH3 + H2O2. Bovine plasma amine oxidase has a molecular weight of 170 kDa and an optimum pH of 6.2 for spermine and 7.2 for spermidine. Amine oxidases are divided into two classes: the pyridoxal and copper containing enzyme to which plasma amine oxidase belongs, and the FAD-containing amino oxidases. Natural substrates include catecholamines, tryptamine derivatives and other physiologically active amines. Plasma amine oxidase is used in research requiring nitrogen group transfers. The molecule is composed of two identical polypeptide chains. There are two pyridoxal phosphates and two atoms of Cu+ per molecule. Bovine plasma amine oxidase is inhibited by copper chelating agents, many carboxyl reagents such as cuprizone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are both non-competitive inhibitors (KI = 30 and 34mM respectively). The assay for determination of amine oxidases employed at Aladdin is essentially that of Tabor et al., JBC, 208, 645, (1954) with the reaction temperature reduced to 25°C. |
备注 | Chromatographically purified through step five of the procedure of Yamada, Y., and Yasunobu, K., JBC, 237, 1511 (1962). A lyophilized powder. (One IU = 4,330 Tabor Units). |
别名 | PAO;SSAO;Amine oxidase(copper-containing);Amine:oxygen oxidoreductase(deaminating) |
个人防护设备 | Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
WGK德国 | 3 |
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