产地：MBI 

货号：EL0012

Applications

Cloning of restriction enzyme generated DNA fragments.

Cloning of PCR products.

Joining of double-stranded oligonucleotide linkers or adaptors to DNA.

Site-directed mutagenesis.

Amplified fragment length polymorphism (AFLP).

Ligase-mediated RNA detection (3).

Nick repair in duplex DNA, RNA or DNA/RNA hybrids.

Self-circularization of linear DNA.

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids (1, 2).

The T4 DNA Ligase requires ATP as cofactor.

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途