订购DNaseIRNasefree;MBIEN0521正品热销,RNA提取纯化

产地：MBI

货号：EN0521

Applications

Preparation of DNA-free RNA (1).

Removal of template DNA following in vitro transcription (1).

Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR (2).

DNA labeling by nick-translation in conjunction with DNA Polymerase I (1).

Studies of DNA-protein interactions by DNase I, RNase-free footprinting (1).

Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used (3).

Description

DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions, see Fig.1:

– in the presence of Mg2+, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion (1);

– in the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides (1).

订购DNaseIRNasefree;MBIEN0521正品热销信息由上海叶舟生物科技有限公司为您提供，如您想了解更多关于订购DNaseIRNasefree;MBIEN0521正品热销报价、型号、参数等信息，欢迎来电或留言咨询。