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MaximaHotStartTaqDNAPolymerase;MBIEP0601正品现货
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
QIAGEN | 一般经销商 | 德国 | 现货 |
产品介绍
EP0601 | 100 u (5 u/µl) | 0.60 ml | 0.60 ml | EP0601 | |
EP0602 | 500 u (5 u/µl) | 2 x 1.25 ml | 2 x 1.25 ml | EP0602 | |
EP0603 | 5 x 500 u (5 u/µl) | 10 x 1.25 ml | 10 x 1.25 ml | EP0603 | |
产地:MBI
货号:EP0601
Applications
Hot start PCR.
High yield amplification of targets up to 3 kb.
RT-PCR.
Description
Maxima® Hot Start Taq DNA Polymerase is designed to enhance the specificity, sensitivity and yield of DNA amplification (1-4). In addition, the enzyme provides the convenience of reaction set-up at room temperature. Maxima® Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification (see Fig.1). The functional activity of the enzyme is restored during a short 4-minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: catalyzes 5'=>3' synthesis of DNA, has no detectable 3'=>5' proofreading exonuclease activity, but possesses 5'=>3' exonuclease activity. It exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Before activation, the two activities are not detectable.
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途