产地：MBI 

货号：ER0111

Conditions for 100% Activity

1X Buffer O:

50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

Bsp68I is supplied in:

10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 10-fold overdigestion with Bsp68I, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.

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