MBIER0202;Cfr42ISacII原装现货
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
暂无 | 暂无 | 暂无 | 现货 |
产地:MBI
货号:ER0202
Conditions for 100% Activity
1X Buffer B:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Cfr42I is supplied in:
10 mM potassium phosphate (pH 7.4 at 25°C), 100 mM NaCl, 1 mM EDTA, 7 mM 2-mercaptoethanol, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Cfr42I, more than 95% of the DNA fragments can be ligated and recut.
Digestion of Agarose-embedded DNA
Minimum 20 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
ER0201 | 1200 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0201 | |
ER0202 | 5 x 1200 u (10 u/µl) | 2 x 1.00 ml | 1.00 ml | ER0202 | |
ER0205 | 2000 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0205 |
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