本文采用TSKgel ODS-100V反相色谱柱建立了中药柴胡的甲醇提取物进行了分析。该方法可用于柴胡六种主要成分的HPLC-ELSD的定量分析。

 

Application No.2: Fast determination of saikosaponins in Bupleurum (柴胡) by rapid resolution liquid chromatography with evaporative light scattering detection

 

 

Condition

Column: TSKgel ODS-100V 3um (4.6mmID x 15cm) (A)

Eluent  A: CH3CN; Eluent B: water

Gradient: 0min(A 30%) →25 min (A 40%)→35 min(A 50%)→45 min(A 100%) Column temperature 25 C

Flow Rate: 1 mL/min

Detection: ELSD(Drift tube temperature for ELSD: 40C Air flow rate: 3.5 Bar)

Temp.: 25 C

Inj. Volume: 20 uL Sample: Extract of Bupleurum by MeOH with 1% pyridine