sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I-sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I

sigma 胶原酶I型 for general use, Type I, 0.25-1.0 FALGPA units/mg solid, >125 CDU/mg solid (CDU = collagen digestion units), essentially salt-free, lyophilized powder
Synonym Clostridiopeptidase A
CAS Number 9001-12-1
Enzyme Commission (EC) Number 3.4.24.3
EG/EC Number 2325829
MDL number MFCD00130830
Descriptions
sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I-sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I

Other Notes View more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer.

Preparation Note Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).

Quality Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.

sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I-sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I

One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.
sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I-sigma 胶原酶I型|Sigma-C-0130 胶原酶I型|Sigma-C-0130|Collagenase I

Properties
suitability release of physiologically active rat hepatocytes tested
storage temp. -20°C
胶原酶I 包装:100mg sigma 分装胶原酶I

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