For Specialized Amino Acid Applications with Postcolumn Derivatization

The AminoPac^®PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

• Resolves phosphorylated and other amino acids by anion-exchange chromatography

• Separates acid-labile amino acids under basic pH conditions

• Separates strongly acidic amino acids

• Requires OPA or ninhydrin postcolumn derivatization

The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

Gradient sepration of amino acid hydrolysate.

Amino Acids by Anion Exchange Using The AminoPac^®PA1 Column.

Anion-Exchange Separation ofO-Phosphorylated Amino Acids Using the AminoPac^®PA1 Column.