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赛默飞Thermo CarboPac PA100 Carbohydrate Column离子色谱IC柱

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赛默飞 一般经销商 美国 现货

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离子色谱IC柱

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产品介绍

Low-Picomole Oligosaccharide Analysis

Oligosaccharides released from therapeutic glycoproteins can differ in size, charge, branching, and linkage. Oligosaccharides with these features can be resolved using the CarboPac®PA100. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection, the PA100 supports detection at low-picomole levels.

  • Separation of closely related oligosaccharides, even isomers, at picomole levels

  • Separation of neutral and charged oligosaccharides in the same run

  • No sample derivatization required for detection

The CarboPac PA100 column is a high-resolution, strong anion-exchange column for enhanced chromatography of oligosaccharides.

The Carbohydrate Membrane Desalter can be used on-line prior to fraction collection.


CarboPac PA100 Column for Oligosaccharide Analysis Specifications
DimensionsCarboPac PA100 Analytical Column:
4×250 mm
CarboPac PA100 Guard Column:
4 × 50 mm
CarboPac PA100 Microbore Column:
2 × 250 mm
CarboPac PA100 Microbore Guard Column:
2 × 50 mm
CarboPac PA100 Semipreparative Column:
9 × 250 mm
CarboPac PA100 Semipreparative Column:
22 × 250 mm
Maximum operating pressure4000 psi (27.9 MPa)
Temperature range4–60 °C
Recommended operating temperature: ambient
Typical operating conditions2400 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min
Mobile phase compatibilitypH 0–14; 100% compatible with common organic solvents.
Ionic form eluents: sodium acetate and sodium hydroxide only.
Resin composition8.5-µm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linking) agglomerated with 275-nm MicroBeadquaternary amine functionalized latex (6% cross-linked).
Anion-exchange capacityApproximately 90 µeq/column (4×250 mm analytical column)
Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.



Separation of neutral oligosaccharide standards. Twelve commonly occurring N-linked neutral oligosaccharides are easily resolved within 20 minutes.

Separation of neutral and sialylated oligosaccharide standards.

Separation of trisialylated oligosaccharides.

Elution of nmol FetuinN-Linked Alditols on the CarboPacPA100 Column.

Comparison of the CarboPacPA1 to PA100 Optimized Conditions.

HPAE-PAD Analysis of Red Hook Amber Beer.


Analytical Columns
CarboPac PA100 Analytical Column (2 x 250 mm)057182
CarboPac PA100 Analytical Column (4 x 250 mm)043055
CarboPac PA100 Column (9 x 250 mm)SP2089
CarboPac PA100 Column (22 x 250 mm)SP2667
Guard Columns
CarboPac PA100 Guard Column (2 x 50 mm)057183
CarboPac PA100 Guard Column (4 x 50 mm)043054


赛默飞Thermo CarboPac PA100 Carbohydrate Column离子色谱IC柱信息由上海希言科学仪器有限公司为您提供,如您想了解更多关于赛默飞Thermo CarboPac PA100 Carbohydrate Column离子色谱IC柱报价、型号、参数等信息,欢迎来电或留言咨询。

注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途

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