pBlueScript II 载体
面议
- 安捷伦
- 生产商
- 进口
- 美国
- 现货
- 全国
- 7
- 1
Agilent pBlueScript II 载体是可用于一系列研究应用的强大克隆载体。这种载体具有广泛的多接头和 21 个独特的限制性内切酶识别位点，适用于各种 DNA 测序和克隆过程。用于分子克隆的质粒载体是噬菌粒（来源于噬菌体的质粒）
Agilent pBlueScript II 载体是可用于一系列研究应用的强大克隆载体。这种载体具有广泛的多接头和 21 个独特的限制性内切酶识别位点，适用于各种 DNA 测序和克隆过程。用于分子克隆的质粒载体是噬菌粒（来源于噬菌体的质粒）
![](https://www.agilent.com/common/requestQuote.jsp?source=contactus>联系我们</a> </li></ul>
</div>
</header>
</section>
<footer>
<a class="pull-right" href="#"> 返回页首<i class="fa fa-chevron-circle-up"></i></a>
</footer>
</section>
<!-- End of features section from the Consumables Product Asset -->
<!--templateName->/SpecificationsSectionPageletTemplate -->
<!--templateName->/SystemComponentsSectionPageletTemplate -->
<!--Time to load systemComponent section : 18--><!--templateName->/TechnologySectionPageletTemplate -->
<!--templateName->/SideBySideSectionPageletTemplate -->
<script type="text/javascript">
var sections = {"section1":{"template":"SideBySideImageTemplate","templateId":"1405227290136_0","id":"section_1405227290136","name":"pBlueScript II Vectors","nameId":"1405227290136_1406546460262","assettype":"Section_C"}};
</script>
<script type="text/javascript">
var howItWorksSection = {"section":{"data":{"attributes_list":"id,home_section_title,section_cta_title,analytics_section_title,section_cta_url,section_links,section_sub_text,component_headline,component_desc","id":"1405227290136","idId":"1405227290136_0","home_section_title":"pBlueScript II Vectors","home_section_titleId":"1405227290136_1406546460262","analytics_section_title":"NoAnalytics","analytics_section_titleId":"1405227290136_1405257770331","assettype":"Section_C","slots":[{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"Plasmids with a phage origin","slot_titleId":"1405227288655_1406546459576","slot_basic_desc":"<p>\r\n\tThe phagemids are cloning vectors to simplify commonly used cloning and sequencing procedures, including nested deletion construction for DNA sequencing, generation of RNA transcripts in vitro, and site-specific mutagenesis and gene mapping. The phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. Flanking the polylinker are T7 and T3 RNA polymerase promoters that can be used to synthesize RNA in vitro. The choice of promoter used to initiate transcription determines the described strand of the insert cloned into the polylinker.</p>\r\n","slot_basic_descId":"1405227288655_1457965536647","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405227288655_1457965621962","image_allign":"Left","image_allignId":"1405227288655_1457965561153","grey_background":"White","grey_backgroundId":"1405227288655_1457965561160","id":"1405227288655","assettype":"Slots_C"},{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_basic_desc":"<p>\r\n\tThe polylinker and T7 and T3 RNA polymerase promoter sequences are present in the N-terminal portion of a lacZ gene fragment. The pBluescript II phagemid has 131 amino acids of ß-galactosidase coding sequence, but the coding sequence is interrupted by the large polylinker. pBluescript II phagemids having no inserts in the polylinker will produce blue colonies in the appropriate strains of bacteria. pBluescript II phagemids that have inserts will produce white colonies using the same strain, because the inserts disrupt the coding region of the lacZ gene fragment.</p>\r\n","slot_basic_descId":"1405227288821_1457965536647","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405227288821_1457965621962","image_allign":"Right","image_allignId":"1405227288821_1457965561153","grey_background":"White","grey_backgroundId":"1405227288821_1457965561160","id":"1405227288821","assettype":"Slots_C"},{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"pBluescript II (+) and (–) are available with two polylinker orientations","slot_titleId":"1405227289976_1406546459576","slot_basic_desc":"<p>\r\n\tKS orientation: Kpn I is nearest the lacZ promoter and Sac I is farthest from the lacZ promoter.</p>\r\n<p>\r\n\tSK orientation: Sac I is the closest to the lacZ promoter and Kpn I is the farthest.</p>\r\n<p>\r\n\tBssH II sites flank T3 and T7 promoters. This 6-base cutter allows insert plus T phage RNA promoters to be excised and gene mapping.</p>\r\n<p>\r\n\tPhagemid can be rescued as ssDNA and contain a 454 bp filamentous f1 phage intergenic region, including the 307 bp origin of replication. The (+) and (–) orientations of the f1 intergenic region allow the rescue of sense or antisense ssDNA by a helper phage.</p>\r\n","slot_basic_descId":"1405227289976_1457965536647","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405227289976_1457965621962","image_allign":"Left","image_allignId":"1405227289976_1457965561153","grey_background":"White","grey_backgroundId":"1405227289976_1457965561160","id":"1405227289976","assettype":"Slots_C"}]}}};
</script>
<section class="item-collection clear pdpSections active operation-block-section">
<header class="col-full operation-block-header">
<div id="howitworks" class="heading" role="heading" aria-level="3">工作原理</div>
</header>
<section class="item col-full operation-block" data-section="pdp:operation">
<div class="media-body" >
<how-it-works></how-it-works>
<script>
var x=document.querySelector(){kind=link}