| 应用 |
Applications for enzyme-labeled (HRP, AP) streptavidin:
Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies
Cell and tissue staining (for light microscopy)
Western blotting (WB) and other membrane-based biotin detection
Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin:
Immunofluorescent assays (IFA)
Tissue staining (for fluorescence microscopy)
Blot immunostaining
Cell staining (incl. fluorescent activated cell sorting) |
| 产品介绍 |
Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups.
These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. |
| 备注 |
单位定义：1单位结合1.0 UG 生物素. 方法描述见文献 GREEN N.M., METHODS IN ENZYMOLOGY, 18, 418 (1970).
来源：Streptomyces avidinii |
| 生化机理 |
reptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. OursStreptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation. |
| 别名 |
抗生物素蛋白链菌素，链亲合素;Streptavidin 来源于阿维丁链霉菌;Streptavidin from Streptomyces avidinii;Streptavidin and Conjugates |
