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Acclaim300C18蛋白质和羧氨酸色谱柱
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
赛默飞 | 一般经销商 | 美国 | 现货 |
Acclaim 300 C18 蛋白质和羧氨酸色谱柱
蛋白质和羧氨酸的高分辨率分离
High-Resolution Separation of Proteins and Peptides
概要:
The Acclaim® 300 features 3 µm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.
- Designed for high-resolution peptide mapping and protein separations
- High-efficiency 3 µm spherical silica substrate
- High-performance bonding chemistry on 300 Å pore silica
- Application tested for suitablility in peptide mapping
- Reproducible for dependable results
- LC/MS compatible
Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions.
The use of a 3 µm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.
规格:
Acclaim PepMap for TFA-Free Peptide Mapping Specifications | |||
Column type | Nano | Capillary | Micro |
Column i.d. | 75 µm | 300 µm | 1.0 mm |
Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
Recommended Flow Rate | 200 nL/min | 4 µL/min | 40 µL/min |
Packing Material | C18, 100 Å,3 µm | C18, 100 Å,3 µm | C18, 100 Å,3 µm |
C18, 300 Å,5 µm | C18, 300 Å,5 µm | C18, 300 Å,5 µm | |
C8, 100 Å,3 µm | C8, 100 Å,3 µm | C8, 100 Å,3 µm | |
C8, 100 Å,5 µm | C8, 100 Å,5 µm | C8, 100 Å,5 µm | |
C4, 300 Å,5 µm | C4, 300 Å,5 µm | C4, 300 Å,5 µm | |
Column | Fused-silica | Glass Lined Tubing | Stainless steel |
Column Inlet | 1/16-in.PEEK sleeve adapter(provided)nanoViper (optional) | 1/16-in.universal fittingnano Viper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in.PEEK tubingnano Viper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
Column Outlet | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in.PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
MS Outlet | 1-m Length |
订购信息:
The standard particle size is nominally 3 µm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters; standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 µm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.
Analytical Columns | |
Acclaim 300, C18, 3 µm Analytical (2.1 x 50 mm) | 60263 |
Acclaim 300, C18, 3 µm Analytical (2.1 x 150 mm) | 60264 |
Acclaim 300, C18, 3 µm (3.0 x 150 mm) | 63684 |
Acclaim 300, C18, 3 µm Analytical (4.6 x 50 mm) | 60265 |
Acclaim 300, C18, 3 µm Analytical (4.6 x 150 mm) | 60266 |
Guard Columns | |
Acclaim 300,C18, 3µm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 | 69690 |
PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA | 69697 |
Hardware | |
Acclaim SST Guard Cartridge Holder V-1 | 59456 |
Guard to Analytical Column Coupler | 59457 |
Guard Kit (Holder and Coupler) | 59526 |
Acclaim SST Guard Cartridge Holder V-2 | 69580 |
Acclaim Guard Kit (Holder and coupler) V-2 | 69707 |
Guard to Analytical Column Coupler V-2 | 74188 |
Micro and Nano Columns | |
Acclaim 300, C18, 3 µm, 300 Å, 75 µm i.d. x 5 cm | 162223 |
Acclaim 300, C18, 3 µm, 300 Å, 75 µm i.d. x 15 cm | 162224 |
Acclaim 300, C18, 3 µm, 300 Å, 300 µm i.d. x 5 cm | 162221 |
Acclaim 300, C18, 3 µm, 300 Å, 300 µm i.d. x 15 cm | 162222 |
Acclaim 120, C18, 3 µm, 120 Å, 1.0 mm i.d. x 5 cm | 162219 |
Acclaim 300, C18, 3 µm, 300 Å, 1.0 mm i.d. x 15 cm | 162220 |
应用谱图:
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途