ACO2 Antibody(N-term)
Cat.#: T0501
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: Q99798
Mol Weight: 85kDa
Size: 100ug

Description
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Recommended Applications
WB, ELISA

Applications and Recommended Dilution Factors
WB: 1:100-500
ELISA: 1:1,000

Species Reactivity
Human, Rat

Products Data  

 

Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (N-term) . Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4
ACO2 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).
ACO2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).

Specificity
This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~160 amino acids from the N-terminal region of human Aconitase.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Alternative Names
ACO2 antibody, ACONM antibody, MGC20605 antibody, MGC33908 antibody, Aconitate hydratase, mitochondrial antibody, Citrate hydro-lyase antibody

Description References
Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004).
Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002).
Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997).
Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993).
Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途