AP2 mu1 Phospho(pT156)(AP2M1)antibody
Cat.#: 3312-1
Rabbit Monoclonal Antibody
Clone ID: EPR4700
Swiss Prot: Q96CW1
Mol Weight: 50kDa
Size: 100ul

Description
Clathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed "assembly proteins," or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115 kDa, denoted AP2 alpha and AP2 beta, a medium chain of 50 kDa, designated AP50, and a small chain (1). The medium chain AP2 mu1 interacts specifically with the tyrosine-based signals of several integral membrane proteins through the consensus sequence YXXPhi, where X can be any residue and Phi is a large hydrophobic residue (2). Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is an important factor for a variety of cellular functions ranging from cell signaling to actin cytoskeletal dynamics and endocytic membrane traffic. It has been shown that the clathrin adaptor complex AP2 acts as a regulator of phosphatidylinositol 4-phosphate 5-kinase (PIPK)-mediated PI(4,5)P(2) synthesis. AP2 directly interacts with the kinase core domain of type I PIPK isozymes via its mu2-subunit in vitro and in native protein extracts (3).

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:50 - 100

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity is based on WB analysis

Products Data

Western blot analysis on 293T cell lysates using anti-Phospho-AP2 mu1 (pT156) RabMAb (cat. #3312-1). Cells were either (A) untreated (B) treated with Calyculin A.

Specificity
A phospho specific peptide corresponding to residues surrounding Threonine 156 of human AP2 mu1 was used as an immunogen. This antibody only detects AP2 mu1 phosphorylated at Threonine 156.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative NamesAP2M1 antibody, AP50 antibody, CLAPM1 antibody, mu2 antibody, AP-2 complex subunit mu antibody, AP-2 mu chain antibody, Adapter-related protein complex 2 mu subunit antibody, Adaptin-mu2 antibody, Adaptor protein complex AP-2 subunit mu antibody, Clathrin assembly protein complex 2 medium chain antibody, Clathrin coat assembly protein AP50 antibody, Clathrin coat-associated protein AP50 antibody, HA2 50 kDa subunit antibody, Plasma membrane adaptor AP-2 50 kDa protein antibody

Description References
1. Druck T, et al. Genomics 30(1):94-7, 1995
2. Follows ER, et al. Biochem J 359(2):427-34, 2001
3. Proc Natl Acad Sci 103(32):11934-9, 2006

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