AMPK alpha 1(PRKAA1)antibody
Cat.#: 1596-1
Rabbit Monoclonal Antibody
Clone ID: Y365
Swiss Prot: Q13131
Mol Weight: 63kDa
Size: 100ul

Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Recommended Applications
WB, IHC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:100-250
IP: 1:50

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis of anti-AMPK alpha-1 RabMAb (cat. # 1596-1), dilution 1:5,000. A: MCF-7 B: Hela
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AMPK alpha RabMAb (cat. # 1596-1).

Specificity
A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit was used as an immunogen

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
PRKAA1 antibody, AMPK antibody, AMPKa1 antibody, MGC33776 antibody, MGC57364 antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, antibody

Description References
1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Stapleton et al. J. Biol. Chem. 271:611-614
3. Hamilton et al. FEBES Lett. 500:163-168, 2001
4. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136

Product References
1. Abiola M, Favier M, Christodoulou-Vafeiadou E, et al, Activation of Wnt/β-Catenin Signaling Increases Insulin Sensitivity through a Reciprocal Regulation of Wnt10b and SREBP-1c in Skeletal Muscle Cells ,PLoS One 4 (2009) e8509
[Application: WB] 

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途