Akt1(AKT1)antibody
Cat.#: 1085-1
Rabbit Monoclonal Antibody
Clone ID: E45
Swiss Prot: P31749
Mol Weight: 60kDa
Size: 100ul

Description
Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:1,000-1:2,500
ICC: 1:1,000
IP: 1:100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

Western blot analysis on NIH3T3 cell lysate using anti- Akt1 pan RabMAb (cat. #1085-1).
Immunohistochemical analysis of paraffin-embedded squamous lung carcinoma using anti-Akt1/PKBa RabMAb (cat. #1085-1).

Specificity
A synthetic peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody can detect Akt1 and Akt3. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AKT1 antibody, AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody

Description References
1. Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599
2. Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318
3. Hajduch, E., et al. Protein kinase B (PKB/Akt)--a key regulator of glucose transport? FEBS Lett. 492: 199
4. Cross, D.A., et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378: 785-789 (1995). 

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