Adducin alpha Phospho(pS481)(ADD1)antibody
Cat.#: 1762-1
Rabbit Monoclonal Antibody
Clone ID: EP733Y
Swiss Prot: P35611
Mol Weight: 130kDa
Size: 100ul

Description
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:250 - 500
ICC: 1:100 -250
IP: 1:50

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on (1) Hela cell lysate and (2) HeLa lysate + TPA using anti-Phospho-Adducin (pS481) RabMAb (catalog #1762-1), dilution 1:20,000.
B. Immunohistochemical analysis of paraffin-embedded human uterus using anti-Adducin alpha Rab MAb. (cat. # 1762-1).

Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 481.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ADD1 antibody, ADDA antibody, MGC3339 antibody, MGC44427 antibody, Alpha-adducin antibody, Erythrocyte adducin subunit alpha antibody

Description References
1. Gardner K, Bennett V., J Biol Chem 261:1339-1348, 1986.
2. Kuhlman PE, et al. J Biol. Chem, 271: 7986-7991, 1996.
3. Dong, LC, et al. J Biol Chem 270: 25534-25540, 1995.
4. Palfrey HC, et al. J Biol Chem. 260:16021-16029, 1985.
5. Fukata, et al. J Cell Biol, 145:347-361, 1995. 

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