14-3-3 sigma(SFN)antibody
Cat.#: 5209-1
Rabbit Monoclonal Antibody
Clone ID: EPR5872
Swiss Prot: P31947
Mol Weight: 28kDa
Size: 100ul

Description
14-3-3 proteins are composed of a highly conserved multigene family of small acidic proteins. Responsive to DNA damage, 14-3-3 sigma expression is induced in a p53-dependent manner. 14-3-3 sigma sequesters cyclin B1-CDC2 complex in cytoplasm, and prevents its entry into nucleus resulting in G2/M cell cycle arrest. Furthermore, 14-3-3 sigma can link to CDK2 and CDK4 and block the cell cycle entry. Thus, 14-3-3 sigma acts as a negative regulator of cell cycle progression and is linked to cancer (1). A keratinocyte-releasable form of 14-3-3 sigma is a potent MMP-1-stimulatory factor in dermal fibroblasts. When it is released from keratinocytes, it induces the expression of MMP1, MMP3, MMP8 and MMP24 in fibroblasts through the p38 mitogen-activated protein kinase (MAPK) signaling pathway (2).

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on (A) fetal lung, (B) A549, (C) human kidney, and (D) human spleen lysates using anti-14-3-3 sigma RabMAb (cat. #5209-1).

Specificity
A synthetic peptide corresponding to residues in human 14-3-3 sigma was used as an immunogen.

Storage Condition and Buffer
Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
SFN antibody, YWHAS antibody, 14-3-3 protein sigma antibody, Epithelial cell marker protein 1 antibody, Stratifin antibody

Description References
1. Wang Z et al. BMC Cancer. 2008 Oct 24;8:308.
2. Ghaffari A et al. J Cell Sci. 2010 Sep 1;123(Pt 17):2996-3005. Epub 2010 Aug 10. 

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途