ACAT(C-term)(CES1)antibody
Cat.#: 1912-1
Rabbit Monoclonal Antibody

Clone ID: EP1376Y
Swiss Prot: P23141
Mol Weight: 60kDa
Size: 100ul

Description
Triacylglycerol hydrolase is a microsomal enzyme that hydrolyzes stored cytoplasmic triacylglycerol in the liver and participates in the lipolysis/re-esterification cycle during the assembly of very-low-density lipoproteins. Expression of human TGH in Escherichia coli yields a protein without enzymatic activity, which suggests that posttranslational processing is necessary for the catalytic activity (1). ACAT esterifies free cholesterol and stores cholesteryl esters in lipid droplets. Macrophage ACAT1 deficiency results in increased atherosclerotic lesion area in hyperlipidemic mice via disrupted cholesterol efflux, increased lipoprotein uptake, accumulation of intracellular vesicles, and accelerated apoptosis (2). Data show that macrophage ACAT1 influences the efflux of both cellular and lipoprotein-derived cholesterol and propose a pathway for the pro-atherogenic transformation of ACAT1(-/-) macrophages (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1: 250 - 500
ICC: 1:50 - 100
IP: 1:100

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

A. Western blot analysis on U937 cell lysate using anti-ACAT RabMAb (cat. # 1912-1), dilution 1:10,000.
B. Immunohistochemical staining of paraffin-embedded human liver using anti-ACAT RabMAb (cat. #1912-1).

Specificity
A synthetic peptide corresponding to residues on the C-terminus of human ACAT was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
CES1 antibody, ACAT antibody, CEH antibody, CES2 antibody, HMSE antibody, HMSE1 antibody, MGC117365 antibody, PCE-1 antibody, SES1 antibody, TGH antibody, Liver carboxylesterase 1 antibody, Serine esterase 1 antibody, Triacylglycerol hydrolase antibody

Description References
1. Alam M, et al. Biochemistry 41(21):6679-87, 2002.
2. Dove DE, et al. Atherosclerosis 186(2):267-74, 2006.
3. Sone DE, et al. Arterioscler Thromb Vasc Biol 25(1): 128-34, 2005. 

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