ATF-1 Phospho(pS63)(ATF1)antibody
Cat.#: 2456-1
Rabbit Monoclonal Antibody
Clone ID: EP1591(2)Y
Swiss Prot: P18846
Mol Weight: 36kDa
Size: 100ul

Description
ATF-1 is a cAMP-responsive enhancer (CRE) binding protein that is expressed in a wide variety of cell types and is capable of dimerizing with CREB. ATF-1 contains a consensus phosphorylation site for PKA and shares extensive homology with CREB in the region surrounding and carboxyl-terminal to the PKA site (1). Epidermal growth factor induction of c-jun expression requires ATF-1 and MEF2 sites in the c-jun promoter. Activation of the c-jun promoter through the ATF-1 site requires phosphorylation of ATF-1 at serine 63. Two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and the ERK pathway acts through downstream phosphorylation of ATF-1 (2).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on HeLa cell lysates using anti-Phospho-ATF-1 (pS63) RabMAb (cat. #2456-1), 1:100,000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-ATF-1 RabMAb (cat. # 2456-1).

Specificity
A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF-1 was used as an immunogen. This antibody detects ATF-1 phosphorylated on serine 63

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATF1 antibody, EWS-ATF1 antibody, FUS/ATF-1 antibody, TREB36 antibody, Cyclic AMP-dependent transcription factor ATF-1 antibody, Activating transcription factor 1 antibody, Protein TREB36 antibody

Description References
1. Rehfuss RP, et al J Biol Chem 266(28):18431-4, 1991
2. Gupta P et al. J Biol Chem 277(52):50550-6, 2002

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