ATF-2 Phospho(pT71)(ATF2)antibody
Cat.#: 1268-1
Rabbit Monoclonal Antibody
Clone ID: E268
Swiss Prot: P15336
Mol Weight: 70kDa
Size: 100ul

Description
ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Recommended Applications
WB, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100
IP: 1:40

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on HeLa cell lysate using anti-Phospho-ATF-2 (pT71) RabMAb (cat. #1268-1); dilution 1:10,000. Cells were (1) untreated, and (2) treated with Anisomycin.
B. Immunofluorescent staining of HeLa cells using anti-Phospho-ATF-2 (pT71) RabMAb (cat. #1268-1).

Specificity
A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF-2 was used as immunogen. The antibody only detects ATF-2 phosphorylated on Threonine 71

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATF2 antibody, CRE-BP1 antibody, CREB2 antibody, HB16 antibody, MGC111558 antibody, TREB7 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, HB16 antibody, cAMP response element-binding protein CRE-BP1 antibody

Description References
1. Maekawa, T., et al. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J. 8: 2023
2. Gupta, S., et al. Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267: 389
3. van Dam, H., et al. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents. EMBO J. 14: 1798
4. Livingstone, C., et al. ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14: 1785

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