现货MBIER1181;Bpu10I原装订购
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
暂无 | 暂无 | 暂无 | 现货 |
产地:MBI
货号:ER1181
Conditions for 100% Activity
1X Buffer Bpu10I:
10 mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Ligation and Recleavage
After 50-fold overdigestion with Bpu10I, more than 90% of the pBR322 DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. No more than 50% of these can be recut due to asymmetric recognition sequence of Bpu10I. The remaining uncleaved ligation products may be cut by Eco81I (SauI) and Bpu1102I (EspI).
Storage Buffer
Bpu10I is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途