现货正品MBIER1321;BspPIAlwI大量订购,其它生化试剂

产地：MBI
货号：ER1321
Conditions for 100% Activity
1X Buffer Tango™:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 55°C.
Ligation and Recleavage
After 10-fold overdigestion with BspPI, approximately 70% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 50% of these can be recut.
Storage Buffer
BspPI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

ER1321	100 u (1-3 u/µl)	1.00 ml	ER1321
ER1322	500 u (1-3 u/µl)	1.00 ml	ER1322

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