现货|PronaseE|Pronase|PronaseE|链霉蛋白酶E|原装热销现货,其它生化试剂

英文名称: Pronase E；Actinase E；Proteinase, streptomyces griseus；Pronase；Proteline；s. Griseus protease；s. Griseus proteinase；Streptomyces griseus protease；Streptomyces griseus proteinase；pronase f. streptomyces griseus
中文名称：链丝菌蛋白酶；链霉菌蛋白酶
CAS号：9036-06-0
来源：From streptomyces griseus
活力(Casein;pH7.4;40℃)：≥4000U/g
pH范围：5.0～9.0
分子量：15000-27000
ROCHE相关产品资料：
Application ： Total degradation of proteins during the isolation of DNA and RNA; (it is not necessary to let pronase self-digest prior to use). Total hydrolysis of proteins in research and for technical purposes. Tissue dissociation in conjunction with collagenase and trypsin. Production of glycopeptides from purified glycoproteins.
Product Description ： Inhibitors: Complete Protease Inhibitor Cocktail Tablets
Optimum pH: 6.0 - 7.5, depending on type of protease activity
Specific activity: approx. 7,000 U/g lyophilizate at 40°C with casein as the substrate, pH 7.5. One unit is the enzyme activity that liberates folin-positive amino acids and peptides, corresponding to 1 µmol Tyr in 1 min under assay conditions.
Specificity: Mixture of several nonspecific endo- and exoproteases that generally digest proteins down to single amino acids. Resolves carboxylic acids and alcohols.
Working concentration: 0.5 - 2 mg/ml
Contents ： Lyophilizate, non-sterile
Contaminants: ≤ 0.002 U DNase/RNase per mg lyophilizate
Note: The preparation contains approximately 20% calcium acetate.
别名：链丝菌蛋白酶E
外观：浅棕色冻干粉
性状：白色或微褐色粉末。为从灰色链霉菌(Streptomyces griseus)中分离出的一种非特异蛋白水解酶(Protease)的专有名称。分子量20000。易溶于盐水和稀盐溶液，最适pH值7.8～8.0
储存条件：4℃
长期保存：-20℃
链霉蛋白酶消化酶的配制
贮存液浓度20mg／m1，-20℃保存。最佳反应缓冲液为0．01mol／LTris—HCl(pH7．8)，0.01mol／LEDTA和0．5％SDS。常用工作浓度lmg／m1，工作温度37℃。
消化酶的选择
在选择用何种酶消化时，应针对要显示的抗原成分不同而选用不同的酶。一般胃蛋白酶和菠萝蛋白酶消化主要用于细胞间质抗原的检测，例如纤连蛋白(fibronectin)、层黏连蛋白(1aminin，LN)和各型胶原的显示；其余酶消化均可用于细胞内抗原的检测。在配制蛋白酶时，应考虑到酶的最佳pH。例如胰蛋白酶的最佳pH为7．6，要在钙离子的激活下于37℃才能达到最佳效率；而胃蛋白酶则在酸性pH值下才能达到最佳效率。另外，经酶消化后的切片应充分洗涤，以去除残留的消化酶，否则会对抗体或组织进行缓慢消化，影响抗原抗体的结合，破坏组织结构。阳性和阴性对照片应同时消化，避免人为差异。
Protease from Streptomyces griseus
(链霉蛋白酶是从链球菌中分离获得的一种丝氨酸蛋白酶和酸性蛋白酶的混合物)
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