产品编号： c8052    
产品名称：  Cholera enterotoxin|Cholera Toxin|Cholergen|霍乱毒素|  
供应商：  Sigma
规格： 0.5mg
目录价： 1985
库存状态： 大量现货
CAS编号：  9012-63-9
应用范围： 科研用
种属来源： Cholera Toxin from Vibrio cholerae 
相关信息： Cholera Toxin from Vibrio cholerae
别名: Cholera enterotoxin, Cholergen
CAS号: 9012-63-9
MDL number: MFCD00130776
包装
Package size based on protein content.
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, ａnd 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Unit Definition
One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine （IBMX）.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide ａnd sodium EDTA
Biochem/physiol Actions
Toxin consisting of an A subunit （27 kDa） surrounded by five B subunits （approximately 12 kDa each）, which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein （Gαs）, reducing GTPase activity ａnd activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
相关类型 Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling ａnd Neuroscience,
G Protein Function, G Proteins ａnd Cyclic Nucleotides, Reagents, Toxins ａnd Venoms
Quality Level PREMIUM
assay ~95% （SDS-PAGE）
form lyophilized powder
composition Protein, ~5% Lowry-TCA 
solubility H2O: soluble10 mg/mL
storage temp. 2-8°C

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途