产地：MBI 

货号：EN0582

Applications

Primer removal from PCR mixtures:

– prior to PCR product sequencing (2),

– for one-tube "megaprimer" PCR mutagenesis (3).

Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.

Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4).

Description

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact, see Fig.1.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1).

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途