MBIEP0161;TerminalDeoxynucleotidylTransferase现货热销,其它生化试剂

产地：MBI

货号：EP0161

Applications

Production of synthetic homo- and heteropolymers (1).

Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus (2, 3).

Oligodeoxyribonucleotide and DNA labeling (2, 4-8).

5'-RACE (Rapid Amplification of cDNA Ends) (9).

In situ localization of apoptosis (10).

Description

Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA (1). TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.

EP0161	500 u (20 u/µl)	0.40 ml	EP0161
EP0162	2500 u (20 u/µl)	2 x 1.00 ml	EP0162

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