Hexokinase;己糖激酶;sigmaH5000原装现货,其它生化试剂

产地：sigma
货号：H5000
Hexokinase from Saccharomyces cerevisiae
别名: ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
CAS号: 9001-51-8
Unit Definition
One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.
Physical form
Lyophilized powder containing approx. 15% sodium citrate
Biochem/physiol Actions
Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
相关类型 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Carbohydrate Metabolism, Enzyme Class Index, General Metabolic Enzymes, Glycolysis, Metabolic Pathways, Metabolomics
type                            Type III
form                           lyophilized powder
foreign activity               glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
storage temp.                   −20°C

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