HRP;Peroxidase;POD 来源于辣根
Peroxidase from horseradish
别名: Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
CAS号: 9003-99-0
Analysis Note
The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
This product is assayed using ABTS for easy comparison to other suppliers′ unit: approx. 1,000 units per mg solid
General description
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
相关类型 1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Analytical Enzymes, Antibody Labeling, Antibody Modification,
Application Index, Armoracia rusticana, Biochemicals Found in Plants - sorted by plant name, Cell Stress, Core Bioreagents, Diagnostic and Analytical Enzymes, Enzyme Class Index, Enzymes, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes I-Z, Immunolabeling, Labeling with Enzymes, Metabolomics, Nutrition Research, Oxidative Stress Enzymes, Peroxidase, Peroxidase Enzymes and Reagents, Research Essentials, Unmodified Horseradish Peroxidase (HRP)
Quality Level PREMIUM
type Type VI-A
form essentially salt-free, lyophilized powder
storage temp. 2-8°C

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