SulforhodamineMultiCaspaseActivityKit
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
Biomol | 暂无 | 暂无 | 现货 |
The Sulforhodamine Multi-Caspase Activity Kit detects apoptosis through the covalent binding of a red fluorescent probe to active caspase enzymes in living cells. The sulforhodamine-labeled caspase inhibitor probe (SR-VAD-FMK; excitation at 550 nm and emission at 595 nm), comprising a sulforhodamine derivative of valylalanylaspartic acid fluoromethyl ketone (VAD-FMK), covalently binds to the reactive cysteine (Cys 285) on the large subunit of active caspase heterodimers. This probe is both cell permeable and non-cytotoxic during the time of the labeling procedure and allows detection of apoptosis by fluorescence microscopy, flow cytometry, and 96-well-plate based fluorometry.
Use of the fluorescence microscope provides a qualitative view of the level of apoptosis induced as compared to the use of a fluorometer or a flow cytometer which will generate quantitative data. One kit includes enough reagent to test 100 samples. The Carboxyfluorescein Multi-Caspase Activity Kit for Apoptosis Detection is a related kit that contains a carboxyfluoroscein-labeled probe (Ex: 490 nm, Em: 520 nm).
Product Specification
Kit/Set Contains: SR-VAD-FMK Reagent, lyophilized, 10X Wash Buffer, Fixative, Hoechst Stain.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途