Fluorogenic substrate for angiotensin converting enzyme-2 (ACE2), a zinc-dependent carboxypeptidase with 42% amino acid sequence identity to ACE1. Unlike Mca-YVADAPK(Dnp), this substrate is not cleaved by caspases. Mca fluorescence is quenched by the Dnp group until cleavage (at Pro-Lys) separates them. Ex: 328 nm, Em: 393 nm, although the following Ex/Em have also been used: 320-340/400-420. This highly quenched (99.6%) substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic Mca control peptide (BML-P127).
Product Specification
Alternative Name: ACE2 substrate (fluorogenic)
MW: 698.2
Purity: 95%
Sequence: Mca-Ala-Pro-Lys(Dnp)-OH  [Mca=(7-methoxycoumarin-4-yl)acetyl;Dnp=2,4-dinitrophenyl]
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
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