ADAMSubstrate(Fluorogenic)原装
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Fluorogenic substrate for ADAM8 (kcat/Km = 1.0 x 105 M-1s-1), ADAM10 (6.2 x 103), ADAM12 (2.8 x 105), TACE (TNF-α converting enzyme, ADAM17) (4.3 x 105), and likely other ADAMs, especially those that cleave TNF-α type sequences. Also efficiently cleaved by MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-13, and MMP-14. FAM fluorescence is quenched by the Dabcyl group until cleavage separates them. Ex.: 485nm, Em.: 530nm.
This substrate is useful for inhibitor screening and kinetic analysis.
Product Specification
MW: 1542.5
Quantity: 1mg net peptide.
Purity: ≥95% (HPLC)
Appearance: Pink powder.
Sequence: Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(5-FAM)-NH2 . TFA (Dabcyl=4-(4-dimethylaminophenylazo)benzoyl; FAM=carboxyfluorescein)
Peptide Content: 70-90%
Solubility: Soluble in DMSO (≥10mM) or TRIS, pH 8.0 assay buffer (≥10μM).
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
Use/Stability: Stock solutions in DMSO are stable for 3 months at -20°C.
Handling: Protect from light and moisture.
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