反相色谱柱TSKgel ODS-100V 分析中药左规丸

本文采用TSKgel ODS-100V反相色谱柱建立了中药左规丸的甲醇提取物进行了分析。该方法可用于左规丸十种主要成分的HPLC-DAD和HPLC-ESI/MS定量分析。

 

Application No.1: A  combined HPLC-PDA and HPLC-MS method for quantitative and qualitative analysis of ten major constituents in the traditional Chinese medicine Zuo Gui Wan (左规丸)

 

 

Condition

 

Column: TSKgel ODS-100V 3um (4.6mmID x 15cm) 

Eluent  A: CH3CN; Eluent B: 0.1%(v/v) formic acid

Gradient: 0min(1%A) →30 min (12%A )→32min(15%A)→55 min(25%A ) → 60 min(40%A ) → 70 min(100%A ) )

Column  temperature: 35 C

Flow Rate: 1 mL/min

Detection: UV 254 nm                     

Inj. Volume: 10 uL Sample: standards for a; Extract of Zuo Gui Wan by methanol for b