TSKgel Amide-80 2um色谱柱在抗体药物糖链分析中的应用
发布时间： 2015-06-01 14:53 来源：东曹（上海）生物科技有限公司
Hydrophilic interaction chromatography (HILIC) with an amide-based stationary phase, used in conjunction with fluorescent-labeled oligosaccharides, is a well-established, robust technique used by many laboratories to obtain high resolution separation of isolated glycans. TSKgel Amide-80 column chemistry is ideally suited for the separation of charged and neutral fractions of glycan pools in a single run.
The application of a TSKgel Amide-80, 2 μm column for the analysis of glycans from monoclonal antibodies is demonstrated in this note, including a comparison to a 3 μm Amide-80 column.