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TSKgel Amide-80 3um色谱柱在糖基化分析(N-聚糖结构解析)中的应用

发布时间: 2016-03-15 13:55 来源:东曹(上海)生物科技有限公司
    Glycosylation is one of the most common forms of post-translational modification of eukaryotic proteins. Glycosylated proteins (glycoproteins) make up the majority of human proteins. The polysaccharide side chains (glycans) play critical roles in physiological and pathological reactions ranging from immunity to cell signaling, development and death. Besides the interest of researchers in characterizing glycosylation patterns of glycoproteins for structure/function analysis, the thorough characterization of glycosylation is a major quality parameter in the production of biotherapeutics.
    Hydrophilic interaction chromatography (HILIC) is a well-recognized technique that effectively separates and quantifies isolated glycans.
下载地址:AN23.pdf