14-3-3 b/a(N-term)(YWHAB)antibody
Cat.#: 1058-1
Rabbit Monoclonal Antibody
Clone ID: E58
Swiss Prot: P31946
Mol Weight: 30kDa
Size: 100ul

Description
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3)

Recommended Applications
WB, IHC, FC

Applications and Recommended Dilution Factors
WB: 1:1,000
IHC: 1:250
FC: 1:50

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data  

A. Western blot analysis of HeLa cell lysate probed with anti-14-3-3 b/a RabMab (Cat#1058-1).
B. Immunohistochemical analysis of human skin using anti-14-3-3 b/a RabMab (cat#1058-1).
C. Flow cytometric analysis of permeabilized HeLa cells using anti-14-3-3 b/a RabMAb (red) (catalog #1058-1) or a rabbit IgG (negative) (green).

Specificity
A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen. Predicted to cross-react with sheep and bovine, based on sequence homology.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
YWHAB antibody, GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, 14-3-3 protein beta/alpha, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody

Description References
1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619

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