14-3-3theta(YWHAQ)antibody
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14-3-3 theta(YWHAQ)antibody
Cat.#: 3887-1
Rabbit Monoclonal Antibody
Clone ID: EPR5123
Swiss Prot: P27348
Mol Weight: 28kDa
Size: 100ul
Description
14-3-3 theta belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and it is 99% identical to the mouse and rat orthologs. 14-3-3 theta is upregulated in patients with amyotrophic lateral sclerosis (1).
Recommended Applications
WB, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
ICC: 1:50 - 100
FC: 1:500 - 1,000
Species Reactivity
Human, Mouse, Rat
Species cross-reactivity is based on WB analysis
Products Data
Western blot analysis on (A) HeLa, (B) 293T, (C) A549, and (D) A431 cell lysates using anti-14-3-3 theta RabMAb (cat. #3887-1).
Flow cytometric analysis of permeablized A549 cells using anti-14-3-3 theta RabMAb (red) (catalog # 3887-1) or a rabbit IgG (negative) (green).
Specificity
A synthetic peptide corresponding to residues on the N-terminus in human 14-3-3 theta was used as an immunogen.
Storage Condition and Buffer
Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
YWHAQ antibody, 14-3-3 antibody, 1C5 antibody, HS1 antibody, 14-3-3 protein theta antibody, 14-3-3 protein T-cell antibody, 14-3-3 protein tau antibody, Protein HS1 antibody
Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途