Adducin alpha(C-term)(ADD1)antibody
Cat.#: 1706-1
Rabbit Monoclonal Antibody
Clone ID: EP734Y
Swiss Prot: P35611
Mol Weight: 130kDa
Size: 100ul

Description
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:5,000-50,000
IHC: 1:250-500
ICC: 1:250-500

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on Hela cell lysate using anti-K562 RabMAb (cat. # 1706-1), dilution 1:50,000.
B. Immunohistochemical analysis of paraffin-embedded human lung using anti-Adducin (C-term) RabMAb (cat. # 1706-1).
C. Immunofluorescent staining of HeLa using anti-Adducin RabMAb (C-term) (cat. # 1706-1).

Specificity
A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ADD1 antibody, ADDA antibody, MGC3339 antibody, MGC44427 antibody, Alpha-adducin antibody, Erythrocyte adducin subunit alpha antibody

Description References
1. Gardner, K.; Bennett, V. : J. Biol. Chem. 261: 1339-1348, 1986.
2. Kuhlman, P.A., C.A. Hughes, V. Bennett, and V.M. Fowler. 1996. J. Biol. Chem. 271: 7986-7991
3. Dong, L., C. Chapline, B. Mousseau, L. Fowler, K. Ramsay, J.L. Stevens, and S. Jaken. 1995. J. Biol. Chem. 270: 25534-25540
4. Palfrey, H. C., Waseem, A. (1985) J. Biol. Chem. 260, 16021-16029
5. Fukata et al. J. Cell Biol., Volume 145, Number 2, April 19, 1999 347-361 

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