Alpha Fodrin(SPTAN1)antibody
Cat.#: 2998-1
Rabbit Monoclonal Antibody

Clone ID: EPR3830
Swiss Prot: Q13813
Mol Weight: 285/150kDa
Size: 100ul

Description
Alpha Fodrin is a member of the spectrin family of widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that is implicated in the establishment of specialized membrane-cytoskeletal domains in differentiating cells (2, 3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells; it may also be induced by the treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of cleavage are amino-terminal and carboxy-terminal fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis (5, 6).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1:500 - 1,000
ICC: 1:250 - 500
IP: 1:10 - 100

Species Reactivity
Human, Rat

Products Data  

 

Western blot analysis on (A) HeLa + camptothecin, (B) HeLa, (C) Jurkat + staurosporine, and (D) Jurkat cell lysates using anti-Alpha Fodrin RabMAb (cat. # 2998-1).
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using anti-Alpha Fodrin RabMAb (cat. # 2998-1).
Immunohistochemical staining of paraffin-embedded human colon tissue using anti-Alpha Fodrin RabMAb (cat. # 2998-1).
 

Immunofluorescent staining of HeLa cells using anti-Alpha Fodrin RabMAb (cat. # 2998-1).

Specificity
A synthetic peptide corresponding to residues in human Alpha Fodrin was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
SPTAN1 antibody, EIEE5 antibody, FLJ17738 antibody, FLJ44613 antibody, NEAS antibody, SPTA2 antibody, Spectrin alpha chain, brain antibody, Alpha-II spectrin antibody, Fodrin alpha chain antibody, Spectrin, non-erythroid alpha chain antibody

Description References
1. Leto TL, et al. Mol Cell Biol. 8(1):1-9, 1988 2. Simonovic M, et al. J Biol Chem. 281(45):34333-40, 2006 3. DH Giebelhaus, et al. The Journal of Cell Biology 105:843- 853,1987 4. Martin SJ, et al. J Biol Chem. 270(12):6425-8, 1995 5. Ulbricht K U, et al. Autoimmunity Reviews 2(2):109- 113,2003 6. Kahaly, G. J., et al. Clinical & Experimental Immunology 140:166-172(7), 2005

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