Alpha-Actinin 4(ACTN4)antibody
Cat.#: 2885-1
Rabbit Monoclonal Antibody
Clone ID: EPR2533(2)
Swiss Prot: O43707
Mol Weight: 100kDa
Size: 100ul

Description
Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm (2). It plays a role in cell motility, invasion, and metastasis of cancer cells (3). Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion (4).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) fetal skeletal muscle, (B) A431, (C) MCF-7, and (D) HeLa cell lysates using anti-Alpha-Actinin 4 RabMAb (cat. # 2885-1).
Immunohistochemical staining of paraffin-embedded human kidney tissue using anti-Alpha-Actinin 4 RabMAb (cat. #2885-1).
Immunofluorescent staining of HeLa cells using anti-Actinin 4 RabMAb (cat. #2885-1).

Specificity
A synthetic peptide corresponding to residues on the N-terminus in human Alpha-Actinin 4 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACTN4 antibody, ACTININ-4 antibody, DKFZp686K23158 antibody, FSGS antibody, FSGS1 antibody, Alpha-actinin-4 antibody, F-actin cross-linking protein antibody, Non-muscle alpha-actinin 4 antibody

Description References
1. Ylänne et al. Structure. 9(7):597-604, 2001. 2. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8. 3. Yamada et al. Int J Oral Maxillofac Surg. 2009. 4. Otey and Carpen. Cell Motil. Cytoskeleton 58:104-111, 2004.

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