AMPK alpha 1 Phospho(pS487)(PRKAA1)antibody
Cat.#: 2802-1
Rabbit Monoclonal Antibody
Clone ID: EPR3051
Swiss Prot: Q13131
Mol Weight: 63kDa
Size: 100ul

Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Recommended Applications
WB, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human

Products Data 

 

Western blot analysis on 293T cell lysates using anti-Phospho-AMPK alpha 1 (pS487) RabMAb (cat. #2802-1). Cells were either (A) untreated (B) treated with LP.
Immunofluorescent staining of HeLa cells using Phospho-specific anti-AMPK RabMAb (cat. # 2802-1).

Specificity
A phospho specific peptide corresponding to residues surrounding Serine 487 of human AMPK alpha 1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
PRKAA1 antibody, AMPK antibody, AMPKa1 antibody, MGC33776 antibody, MGC57364 antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, antibody

Description References
1. Mitchelhill, K. I., et al. J. Biol. Chem. 269, 2361-2364, 1994 2. Stapleton et al. J. Biol. Chem. 271:611-614 3. Hamilton et al. FEBES Lett. 500:163-168, 2001 4. Carling, D., et al. Eur. J. Biochem. 186, 129-136, 1989 

AMPKalpha1Phospho(pS487)(PRKAA1)antibody信息由上海希美生物科技有限公司为您提供，如您想了解更多关于AMPKalpha1Phospho(pS487)(PRKAA1)antibody报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途