AMPK gamma-1(C-term)(PRKAG1)antibody
Cat.#: 1592-1
Rabbit Monoclonal Antibody
Clone ID: Y308
Swiss Prot: P54619
Mol Weight: 38kDa
Size: 100ul

Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Recommended Applications
WB, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000
IP: 1:50
FC: 1:20

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on Jurkat cell lysate using anti-AMPK gamma-1 (C-term) RabMAb (cat. # 1592-1), dilution 1:1,000.

Specificity
A synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit was used as an immunogen

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
PRKAG1 antibody, AMPKG antibody, MGC8666 antibody, 5'-AMP-activated protein kinase subunit gamma-1 antibody, antibody

Description References
1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136
3. Carling, D., Zammit, V. A,, and Hardie, D. G. (1987) FEES Lett. 223,217-222

Product References
1. Anderson KA, Ribar TJ, Lin F, et al, Hypothalamic CaMKK2 Contributes to the Regulation of Energy Balance ,Cell Metab 7 (2008) 377-388
[Application: WB]

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