Atg12(ATG12)antibody
Cat.#: 3473-1
Rabbit Monoclonal Antibody
Clone ID: EPR4799
Swiss Prot: O94817
Mol Weight: 22/55kDa
Size: 100ul

Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the ATG12-ATG5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:50-100
ICC: 1:100-250
IP: 1:10-100
FC: 1:10-100

Species Reactivity
Human

Species cross-reactivity is based on WB analysis

Products Data 

 

Western blot analysis on (A) THP-1, (B) HepG2, (C) Raji, and (D) fetal kidney lysates using anti-Atg12 RabMAb (cat. #3473-1).
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 RabMAb (cat. #3473-1).
Western blot analysis on (A) Atg12 transfected 293T and (B) 293T lysates using anti-Atg12 RabMAb (cat. #3473-1).

Specificity
A synthetic peptide corresponding to residues in human Atg12 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATG12 antibody, APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Ubiquitin-like protein ATG12 antibody, Autophagy-related protein 12 antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. Phillips AR et al. Genetics. 2008 Mar;178(3):1339-53.
3. Williams RA et al. Autophagy. 2009 Feb;5(2):159-72.

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