ATM antibody
Cat.#: 1549-1
Rabbit Monoclonal Antibody
Clone ID: Y170
Swiss Prot: Q13315
Mol Weight: 370kDa
Size: 100ul

Description
ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomal recessive disorder characterized by cerebellar ataxia, immune deficiencies, increased cancer predisposition, chromosomal instability and radiation sensitivity (1-2). This large protein (370 kDa) is involved in genome stability, cellular responses to DNA damage and cell cycle control. More than 100 mutations have been identified so far and are expected to inactivate the ATM protein by truncation or large deletions (3). Defects in ATM contribute to various B cell and T cell Leukemia (4).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100-250
ICC: 1:100-250
IP: 1:50

Species Reactivity
Human

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data  

 

A. Western blot analysis on 293 cell lysate using anti-ATM RabMAb (cat. # 1549-1) dilution 1:5,000.
B. Immunohistochemical analysis of paraffin-embedded human carcinoma using anti-ATM RabMAb (cat. # 1549-1).
C. Immunofluorescent staining of 293 cells using anti-ATM RabMAb (cat. # 1549-1).

Specificity
A synthetic peptide corresponding to residues surrounding Serine 1981 of human ATM was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATM antibody, AT1 antibody, ATA antibody, ATC antibody, ATD antibody, ATDC antibody, ATE antibody, DKFZp781A0353 antibody, MGC74674 antibody, TEL1 antibody, TELO1 antibody, Serine-protein kinase ATM antibody, Ataxia telangiectasia mutated antibody

Description References
1. Harnden, D.G. (1994) Int. J. Radiat. Biol. 66, S13-S19
2. Lavin, M.F. and Shiloh, Y. (1997) Ann. Rev. Immunol. 15, 177-202
3. Savitsky, K., Sfez, S., Tagle, D.A., Ziv, Y., Sartiel, A., Collins, F.S., Shiloh, Y. and Rotman, G. (1995) Hum. Mol. Gen. 4, 2025-2032.
4. Stankovic T., Weber P., Stewart G., Bedenham T., Murray J., Byrd P.J., Moss P.A.H., Taylor A.M.R.; Lancet 353:26-29(1999).

Product References
1. Gorodetsky E, Calkins S , Ahn J, et al, ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain ,DNA repair 6 ( 2007 ) 1698–1707
[Application: ICC]
2. Bitona S, Gropp M, Itsyksonb P, et al, ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells ,DNA repair 6 (2007) 128–134
[Application: ICC]
3. Li H, Baskaran R, Krisky DM, et al, Chk2 is required for HSV-1 ICP0-mediated G2/M arrest and enhancement of virus growth ,Virology 375 (2008) 13–23
[Application: WB]
4. Schwartz RA, Carson CT, Schuberth C, et al, Adeno-Associated Virus Replication Induces a DNA Damage Response Coordinated by DNA-Dependent Protein Kinase ,J. Virol. 83 (2009) 6269 -6278
[Application: IP]
5. Lakdawala SS, Schwartz RA, Ferenchak K, et al, Differential Requirements of the C Terminus of Nbs1 in Suppressing Adenovirus DNA Replication and Promoting Concatemer Formation ,Journal of Virology 82 (2008) 8362–8372
[Application: WB]
6. Biton S, Dar I, Mittelman L, et al, Nuclear Ataxia-Telangiectasia Mutated (ATM) Mediates the Cellular Response to DNA Double Strand Breaks in Human Neuron-like Cells ,The Journal of Biological Chemistry 281 (2006) 17482–17491
[Application: ICC]
7. Cheng WH, Muftic D, Muftuoglu M, et al, WRN is required for ATM activation and the S-phase checkpoint in response to interstrand crosslink-induced DNA double strand breaks ,Mol. Biol. Cell 19 (2008) 3923 - 3933
[Application: WB, ICC]
8. Togano T, Sasaki M, Watanabe M, et al, Induction of oncogene addiction shift to NF-κB by camptothecin in solid tumor cells ,Biochem Biophys Res Commun 390 (2009) 60-64
[Application: WB]
9. Wu M, Kang MM, Schoene NW, et al, Selenium Compounds Activate Early Barriers of Tumorigenesis ,J Biol Chem 285 (2010) 12055-12062
10. Boichuk S, Hu L, Hein J, et al, Multiple DNA Damage Signaling and Repair Pathways Deregulated by Simian Virus 40 Large T Antigen ,J Virol 84 (2010) 8007-8020
11. Qi Y, Schoene NW, Lartey FM, et al, Selenium Compounds Activate ATM-dependent DNA Damage Response via the Mismatch Repair Protein hMLH1 in Colorectal Cancer Cells ,J Biol Chem 285 (2010) 33010-33017

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