ACTG1 Antibody(N-term)
Cat.#: T1450
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P63261
Mol Weight: 42kDa
Size: 100ug

Description
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
IHC: 1:50~100
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of ACTG1 antibody (N-term) in HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ACTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41~71 amino acids from the N-terminal region of human ACTG1.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names
ACTG1 antibody, ACT antibody, ACTG antibody, DFNA20 antibody, DFNA26 antibody, Actin, cytoplasmic 2, N-terminally processed antibody, Gamma-actin antibody

Description References
de Heer,A.M., Ann. Otol. Rhinol. Laryngol. 118 (5), 382-390 (2009)
Mouilleron,S., EMBO J. 27 (23), 3198-3208 (2008)
Liu,P., J Genet Genomics 35 (9), 553-558 (2008) 

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途